J - Cyclodextrin / Diol Phase Silica Analysis of Drug Enantiomers in Liquid Chromatography Materials for Serum by Direct Injection

13-Cyclodextrin (CD)/diol phase silica materials have been designed for direct serum injection assays of drug enantiomers. The mixed functional phase (MFP) materials having /3-CD and diol phases were synthesized in four steps: introduction of a 3-chloropropyl phase, introduction of a /3-CD phase by ether linkage, introduction of a 3glycidoxypropyl phase and hydrolysis of the oxirange ring to a diol phase. The obtained MFP materials were more stable against continuous flow of an eluent including phosphate buffer and organic solvent and! or acid hydrolysis than only $-CD-bonded materials were. These MFP materials could be used for direct serum injection assays of drug enantiomers. The recovery of drug enantiomers from serum was almost 100%.